Thymol, a naturally occurring monocyclic dietary phenolic compound protects Chinese hamster lung fibroblasts from radiation-induced cytotoxicity

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Abstract

The effect of thymol (TOH), a dietary compound was investigated for its ability to protect against radiation-induced cytotoxicity in Chinese hamster lung fibroblast (V79) cells growing in vitro. Treatment of V79 cells with 25 μg/ml of TOH prior to 10 Gy gamma radiation resulted increase in the cell viability than that of radiation alone as evaluated by MTT assay. Similarly, there was a significant increase in the surviving fraction observed with 25 μg/ml of TOH administered 1 h prior to graded doses of gamma radiation. Further, 25 μg/ml TOH treatment before irradiation significantly decreased the percentage of radiation-induced apoptotic cells (sub-G1 population) analyzed by flow cytometry as well as DNA ladder assay. TOH was found to inhibit various free radicals generated in vitro, viz., DPPH, O2{radical dot}, ABTS{radical dot}+ and OH{radical dot} in a concentration dependent manner. TOH also inhibited the radiation-induced decrease in intracellular glutathione, superoxide dismutase and catalase enzyme levels in V79 cells accompanied by the reduction in lipid peroxides. Our study demonstrated antagonistic potential of TOH against radiation-induced oxidative stress, lipid peroxidation resulting in increased cell viability.

Original languageEnglish
Pages (from-to)70-77
Number of pages8
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume680
Issue number1-2
DOIs
Publication statusPublished - 11-2009

All Science Journal Classification (ASJC) codes

  • Genetics
  • Health, Toxicology and Mutagenesis

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