Thymol, a naturally occurring monocyclic dietary phenolic compound protects Chinese hamster lung fibroblasts from radiation-induced cytotoxicity

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Abstract

The effect of thymol (TOH), a dietary compound was investigated for its ability to protect against radiation-induced cytotoxicity in Chinese hamster lung fibroblast (V79) cells growing in vitro. Treatment of V79 cells with 25 μg/ml of TOH prior to 10 Gy gamma radiation resulted increase in the cell viability than that of radiation alone as evaluated by MTT assay. Similarly, there was a significant increase in the surviving fraction observed with 25 μg/ml of TOH administered 1 h prior to graded doses of gamma radiation. Further, 25 μg/ml TOH treatment before irradiation significantly decreased the percentage of radiation-induced apoptotic cells (sub-G1 population) analyzed by flow cytometry as well as DNA ladder assay. TOH was found to inhibit various free radicals generated in vitro, viz., DPPH, O2{radical dot}, ABTS{radical dot}+ and OH{radical dot} in a concentration dependent manner. TOH also inhibited the radiation-induced decrease in intracellular glutathione, superoxide dismutase and catalase enzyme levels in V79 cells accompanied by the reduction in lipid peroxides. Our study demonstrated antagonistic potential of TOH against radiation-induced oxidative stress, lipid peroxidation resulting in increased cell viability.

Original languageEnglish
Pages (from-to)70-77
Number of pages8
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume680
Issue number1-2
DOIs
Publication statusPublished - 11-2009

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Thymol
Cricetulus
Fibroblasts
Radiation
Lung
Gamma Rays
Cell Survival
Radiation Dosage
Lipid Peroxides
Catalase
Lipid Peroxidation
Superoxide Dismutase
Free Radicals
Glutathione
Flow Cytometry
Oxidative Stress
DNA
Enzymes
Population

All Science Journal Classification (ASJC) codes

  • Genetics
  • Health, Toxicology and Mutagenesis

Cite this

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title = "Thymol, a naturally occurring monocyclic dietary phenolic compound protects Chinese hamster lung fibroblasts from radiation-induced cytotoxicity",
abstract = "The effect of thymol (TOH), a dietary compound was investigated for its ability to protect against radiation-induced cytotoxicity in Chinese hamster lung fibroblast (V79) cells growing in vitro. Treatment of V79 cells with 25 μg/ml of TOH prior to 10 Gy gamma radiation resulted increase in the cell viability than that of radiation alone as evaluated by MTT assay. Similarly, there was a significant increase in the surviving fraction observed with 25 μg/ml of TOH administered 1 h prior to graded doses of gamma radiation. Further, 25 μg/ml TOH treatment before irradiation significantly decreased the percentage of radiation-induced apoptotic cells (sub-G1 population) analyzed by flow cytometry as well as DNA ladder assay. TOH was found to inhibit various free radicals generated in vitro, viz., DPPH, O2{radical dot}, ABTS{radical dot}+ and OH{radical dot} in a concentration dependent manner. TOH also inhibited the radiation-induced decrease in intracellular glutathione, superoxide dismutase and catalase enzyme levels in V79 cells accompanied by the reduction in lipid peroxides. Our study demonstrated antagonistic potential of TOH against radiation-induced oxidative stress, lipid peroxidation resulting in increased cell viability.",
author = "Archana, {P. R.} and {Nageshwar Rao}, B. and Mamatha Ballal and {Satish Rao}, {B. S.}",
year = "2009",
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T1 - Thymol, a naturally occurring monocyclic dietary phenolic compound protects Chinese hamster lung fibroblasts from radiation-induced cytotoxicity

AU - Archana, P. R.

AU - Nageshwar Rao, B.

AU - Ballal, Mamatha

AU - Satish Rao, B. S.

PY - 2009/11

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N2 - The effect of thymol (TOH), a dietary compound was investigated for its ability to protect against radiation-induced cytotoxicity in Chinese hamster lung fibroblast (V79) cells growing in vitro. Treatment of V79 cells with 25 μg/ml of TOH prior to 10 Gy gamma radiation resulted increase in the cell viability than that of radiation alone as evaluated by MTT assay. Similarly, there was a significant increase in the surviving fraction observed with 25 μg/ml of TOH administered 1 h prior to graded doses of gamma radiation. Further, 25 μg/ml TOH treatment before irradiation significantly decreased the percentage of radiation-induced apoptotic cells (sub-G1 population) analyzed by flow cytometry as well as DNA ladder assay. TOH was found to inhibit various free radicals generated in vitro, viz., DPPH, O2{radical dot}, ABTS{radical dot}+ and OH{radical dot} in a concentration dependent manner. TOH also inhibited the radiation-induced decrease in intracellular glutathione, superoxide dismutase and catalase enzyme levels in V79 cells accompanied by the reduction in lipid peroxides. Our study demonstrated antagonistic potential of TOH against radiation-induced oxidative stress, lipid peroxidation resulting in increased cell viability.

AB - The effect of thymol (TOH), a dietary compound was investigated for its ability to protect against radiation-induced cytotoxicity in Chinese hamster lung fibroblast (V79) cells growing in vitro. Treatment of V79 cells with 25 μg/ml of TOH prior to 10 Gy gamma radiation resulted increase in the cell viability than that of radiation alone as evaluated by MTT assay. Similarly, there was a significant increase in the surviving fraction observed with 25 μg/ml of TOH administered 1 h prior to graded doses of gamma radiation. Further, 25 μg/ml TOH treatment before irradiation significantly decreased the percentage of radiation-induced apoptotic cells (sub-G1 population) analyzed by flow cytometry as well as DNA ladder assay. TOH was found to inhibit various free radicals generated in vitro, viz., DPPH, O2{radical dot}, ABTS{radical dot}+ and OH{radical dot} in a concentration dependent manner. TOH also inhibited the radiation-induced decrease in intracellular glutathione, superoxide dismutase and catalase enzyme levels in V79 cells accompanied by the reduction in lipid peroxides. Our study demonstrated antagonistic potential of TOH against radiation-induced oxidative stress, lipid peroxidation resulting in increased cell viability.

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