Total phenolic content and free radical scavenging activity of Salacia oblonga and Gymnema Sylvestre: An in vitro comparative study

Shivani Uday, Bheemesh Vangalapati, Poornima A. Manjrekar, Anupama Hegde

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Abstract

The over production of free radicals is involved in triggering many diseases such as diabetes, cancer, rheumatoid arthritis, cirrhosis and arteriosclerosis. Antioxidants derived from plant sources offer resistance against the oxidative stress by way of their free radicals scavenging & lipid peroxidation inhibitory actions. Present study was designed to compare the free radical scavenging activity in relation to the total phenolic content of G. sylvestre & S. oblonga. Total phenolic content of the extracts was determined by using Folin-Ciocalteu method following a slightly modified method of Singleton by comparing with standard Gallic acid. The ability to scavenge 1, l-diphenyl-2-picryl-hydrazyl (DPPH) radical is measured by a decrease in the absorbance at 517 nm as described by Hou comparing with standard Ascorbic acid. Phenolic contents ranging from 35 to 175 and 30 to 85mg of Gallic acid equivalents (GAE) per gram of extract were present in S.oblonga & G.sylvestre respectively. At 100ug concentration in S.oblonga & G.sylvestre produced 81.7 & 66.8% of DPPH scavenging activity respectively and the standard ascorbic acid (10μg) produced 91.5% scavenging activity. Based on the study results, both the plant extracts of S.oblonga and G.sylvestre have significant amounts of phenolic compounds and exhibit free radical scavenging potential. However, between the two, S.oblonga plant had better antioxidant properties. Hence these herbal constituents are of adjuvant importance in the management of various free-radical mediated pathological conditions.

Original languageEnglish
Article number54
Pages (from-to)270-273
Number of pages4
JournalInternational Journal of Pharmaceutical Sciences Review and Research
Volume33
Issue number2
Publication statusPublished - 01-01-2015

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Salacia
Gymnema sylvestre
Free Radicals
Gallic Acid
Ascorbic Acid
Antioxidants
Arteriosclerosis
Plant Extracts
Lipid Peroxidation
Rheumatoid Arthritis
Oxidative Stress
Fibrosis
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

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title = "Total phenolic content and free radical scavenging activity of Salacia oblonga and Gymnema Sylvestre: An in vitro comparative study",
abstract = "The over production of free radicals is involved in triggering many diseases such as diabetes, cancer, rheumatoid arthritis, cirrhosis and arteriosclerosis. Antioxidants derived from plant sources offer resistance against the oxidative stress by way of their free radicals scavenging & lipid peroxidation inhibitory actions. Present study was designed to compare the free radical scavenging activity in relation to the total phenolic content of G. sylvestre & S. oblonga. Total phenolic content of the extracts was determined by using Folin-Ciocalteu method following a slightly modified method of Singleton by comparing with standard Gallic acid. The ability to scavenge 1, l-diphenyl-2-picryl-hydrazyl (DPPH) radical is measured by a decrease in the absorbance at 517 nm as described by Hou comparing with standard Ascorbic acid. Phenolic contents ranging from 35 to 175 and 30 to 85mg of Gallic acid equivalents (GAE) per gram of extract were present in S.oblonga & G.sylvestre respectively. At 100ug concentration in S.oblonga & G.sylvestre produced 81.7 & 66.8{\%} of DPPH scavenging activity respectively and the standard ascorbic acid (10μg) produced 91.5{\%} scavenging activity. Based on the study results, both the plant extracts of S.oblonga and G.sylvestre have significant amounts of phenolic compounds and exhibit free radical scavenging potential. However, between the two, S.oblonga plant had better antioxidant properties. Hence these herbal constituents are of adjuvant importance in the management of various free-radical mediated pathological conditions.",
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