Abstract
Background: Based on the reported antioxidant and anti-inflammatory potential of Terminalia paniculata, the bark aqueous extract (TPW) was investigated against liver damage.Methods: Intrinsic cytotoxicity was tested on normal human liver (Chang) cell lines, followed by acute and sub-chronic toxicity studies in mice. TPW was then evaluated against CCl4-induced liver toxicity in rats. Liver enzymes (AST, ALT, and ALP) and antioxidant markers were assessed. The effect of TPW on isolated hepatic cells, post-CCl4 administration, was assessed by isolated mitochondrial membrane staining. The actions of TPW on apoptotic pathway in CCl4-treated Chang cells were also elucidated.Results: TPW was found to be safe at all doses tested in both in vitro and in vivo toxicity studies. TPW (400 mg/kg, p.o.) significantly (*p <0.05) improved liver enzyme activity as compared to CCl4. Also, it improved antioxidant status (GSH, GST, MDA and total thiol) and preserved hepatic cell architecture. TPW pre-treatment significantly attenuated the levels of phospho-p53, p53, cleaved caspase-3, phospho-Bad, Bad and cleaved PARP in CCl4-treated Chang cells, improving the viability considerably.Conclusion: The findings support a protective role for Terminalia paniculata in pathologies involving oxidative stress.
| Original language | English |
|---|---|
| Article number | 127 |
| Journal | BMC Complementary and Alternative Medicine |
| Volume | 13 |
| DOIs | |
| Publication status | Published - 06-06-2013 |
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All Science Journal Classification (ASJC) codes
- Complementary and alternative medicine
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Toxicological evaluation of Terminalia paniculata bark extract and its protective effect against CCl4-induced liver injury in rodents. / Talwar, Sahil; Jagani, Hitesh V.; Nayak, Pawan G.; Kumar, Nitesh; Kishore, Anoop; Bansal, Punit; Shenoy, Rekha R.; Nandakumar, Krishnadas.
In: BMC Complementary and Alternative Medicine, Vol. 13, 127, 06.06.2013.Research output: Contribution to journal › Article
TY - JOUR
T1 - Toxicological evaluation of Terminalia paniculata bark extract and its protective effect against CCl4-induced liver injury in rodents
AU - Talwar, Sahil
AU - Jagani, Hitesh V.
AU - Nayak, Pawan G.
AU - Kumar, Nitesh
AU - Kishore, Anoop
AU - Bansal, Punit
AU - Shenoy, Rekha R.
AU - Nandakumar, Krishnadas
PY - 2013/6/6
Y1 - 2013/6/6
N2 - Background: Based on the reported antioxidant and anti-inflammatory potential of Terminalia paniculata, the bark aqueous extract (TPW) was investigated against liver damage.Methods: Intrinsic cytotoxicity was tested on normal human liver (Chang) cell lines, followed by acute and sub-chronic toxicity studies in mice. TPW was then evaluated against CCl4-induced liver toxicity in rats. Liver enzymes (AST, ALT, and ALP) and antioxidant markers were assessed. The effect of TPW on isolated hepatic cells, post-CCl4 administration, was assessed by isolated mitochondrial membrane staining. The actions of TPW on apoptotic pathway in CCl4-treated Chang cells were also elucidated.Results: TPW was found to be safe at all doses tested in both in vitro and in vivo toxicity studies. TPW (400 mg/kg, p.o.) significantly (*p <0.05) improved liver enzyme activity as compared to CCl4. Also, it improved antioxidant status (GSH, GST, MDA and total thiol) and preserved hepatic cell architecture. TPW pre-treatment significantly attenuated the levels of phospho-p53, p53, cleaved caspase-3, phospho-Bad, Bad and cleaved PARP in CCl4-treated Chang cells, improving the viability considerably.Conclusion: The findings support a protective role for Terminalia paniculata in pathologies involving oxidative stress.
AB - Background: Based on the reported antioxidant and anti-inflammatory potential of Terminalia paniculata, the bark aqueous extract (TPW) was investigated against liver damage.Methods: Intrinsic cytotoxicity was tested on normal human liver (Chang) cell lines, followed by acute and sub-chronic toxicity studies in mice. TPW was then evaluated against CCl4-induced liver toxicity in rats. Liver enzymes (AST, ALT, and ALP) and antioxidant markers were assessed. The effect of TPW on isolated hepatic cells, post-CCl4 administration, was assessed by isolated mitochondrial membrane staining. The actions of TPW on apoptotic pathway in CCl4-treated Chang cells were also elucidated.Results: TPW was found to be safe at all doses tested in both in vitro and in vivo toxicity studies. TPW (400 mg/kg, p.o.) significantly (*p <0.05) improved liver enzyme activity as compared to CCl4. Also, it improved antioxidant status (GSH, GST, MDA and total thiol) and preserved hepatic cell architecture. TPW pre-treatment significantly attenuated the levels of phospho-p53, p53, cleaved caspase-3, phospho-Bad, Bad and cleaved PARP in CCl4-treated Chang cells, improving the viability considerably.Conclusion: The findings support a protective role for Terminalia paniculata in pathologies involving oxidative stress.
UR - http://www.scopus.com/inward/record.url?scp=84878488721&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84878488721&partnerID=8YFLogxK
U2 - 10.1186/1472-6882-13-127
DO - 10.1186/1472-6882-13-127
M3 - Article
C2 - 23742226
AN - SCOPUS:84878488721
VL - 13
JO - BMC Complementary and Alternative Medicine
JF - BMC Complementary and Alternative Medicine
SN - 1472-6882
M1 - 127
ER -