Transitional metal ions induced damage to biomolecules

Role of ferryl and perferryl radicals

Mungli Prakash, Sharmila Upadhya

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the effect of oxidized transitional metal (ferric and cupric) ions on the amino acids. Methods: 25 mmol/L hydroxyproline and 25 mmol/L histidine were incubated with 50 μL Fe3+ and Cu2+ ions at pH 7.4 and 37 °C for 30 mins in separate test tubes. Then 500 μL of 1% thiobarbituricacid (TBA) was added to the incubated amino acids followed by addition of 500 μL of glacial acetic acid. The resultant mixture was vortexed and heated at 100 °C for 30 min. Absorbance readings were noted after cooling to room temperature. The experiment was repeated in the presence of various reagents, like hydroxyl radical scavengers, antioxidant enzymes, and reducing agents and metal ion chelators. Results: The pink chromogen formed with the absorbance maxima at 524 nm, AND shifted to 560 nm in alkaline pH. The absorbance was expressed as TBA-adduct in MDA units. The TBA-adduct decreased in the presence of reducing agents and metal ion chelators. Antioxidant enzymes and hydroxyl radical scavengers did not show any effect. Conclusion: Transitional metal ions in their oxidized state showed significant damage to amino acids, hydroxyproline and histidine. The results indicate the possible role played by high-valent oxo-iron species, ferryl and perferry radicals in damaging biomolecules.

Original languageEnglish
Pages (from-to)32-35
Number of pages4
JournalAsian Pacific Journal of Tropical Medicine
Volume3
Issue number1
DOIs
Publication statusPublished - 01-01-2010
Externally publishedYes

Fingerprint

Metals
Ions
Hydroxyproline
Reducing Agents
Chelating Agents
Histidine
Amino Acids
Hydroxyl Radical
Antioxidants
Enzymes
Acetic Acid
Reading
Temperature

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

@article{eda220cfc79e460ea592b68ad6e3a0ee,
title = "Transitional metal ions induced damage to biomolecules: Role of ferryl and perferryl radicals",
abstract = "Objective: To investigate the effect of oxidized transitional metal (ferric and cupric) ions on the amino acids. Methods: 25 mmol/L hydroxyproline and 25 mmol/L histidine were incubated with 50 μL Fe3+ and Cu2+ ions at pH 7.4 and 37 °C for 30 mins in separate test tubes. Then 500 μL of 1{\%} thiobarbituricacid (TBA) was added to the incubated amino acids followed by addition of 500 μL of glacial acetic acid. The resultant mixture was vortexed and heated at 100 °C for 30 min. Absorbance readings were noted after cooling to room temperature. The experiment was repeated in the presence of various reagents, like hydroxyl radical scavengers, antioxidant enzymes, and reducing agents and metal ion chelators. Results: The pink chromogen formed with the absorbance maxima at 524 nm, AND shifted to 560 nm in alkaline pH. The absorbance was expressed as TBA-adduct in MDA units. The TBA-adduct decreased in the presence of reducing agents and metal ion chelators. Antioxidant enzymes and hydroxyl radical scavengers did not show any effect. Conclusion: Transitional metal ions in their oxidized state showed significant damage to amino acids, hydroxyproline and histidine. The results indicate the possible role played by high-valent oxo-iron species, ferryl and perferry radicals in damaging biomolecules.",
author = "Mungli Prakash and Sharmila Upadhya",
year = "2010",
month = "1",
day = "1",
doi = "10.1016/S1995-7645(10)60027-2",
language = "English",
volume = "3",
pages = "32--35",
journal = "Asian Pacific Journal of Tropical Medicine",
issn = "1995-7645",
publisher = "Elsevier (Singapore) Pte Ltd",
number = "1",

}

Transitional metal ions induced damage to biomolecules : Role of ferryl and perferryl radicals. / Prakash, Mungli; Upadhya, Sharmila.

In: Asian Pacific Journal of Tropical Medicine, Vol. 3, No. 1, 01.01.2010, p. 32-35.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Transitional metal ions induced damage to biomolecules

T2 - Role of ferryl and perferryl radicals

AU - Prakash, Mungli

AU - Upadhya, Sharmila

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Objective: To investigate the effect of oxidized transitional metal (ferric and cupric) ions on the amino acids. Methods: 25 mmol/L hydroxyproline and 25 mmol/L histidine were incubated with 50 μL Fe3+ and Cu2+ ions at pH 7.4 and 37 °C for 30 mins in separate test tubes. Then 500 μL of 1% thiobarbituricacid (TBA) was added to the incubated amino acids followed by addition of 500 μL of glacial acetic acid. The resultant mixture was vortexed and heated at 100 °C for 30 min. Absorbance readings were noted after cooling to room temperature. The experiment was repeated in the presence of various reagents, like hydroxyl radical scavengers, antioxidant enzymes, and reducing agents and metal ion chelators. Results: The pink chromogen formed with the absorbance maxima at 524 nm, AND shifted to 560 nm in alkaline pH. The absorbance was expressed as TBA-adduct in MDA units. The TBA-adduct decreased in the presence of reducing agents and metal ion chelators. Antioxidant enzymes and hydroxyl radical scavengers did not show any effect. Conclusion: Transitional metal ions in their oxidized state showed significant damage to amino acids, hydroxyproline and histidine. The results indicate the possible role played by high-valent oxo-iron species, ferryl and perferry radicals in damaging biomolecules.

AB - Objective: To investigate the effect of oxidized transitional metal (ferric and cupric) ions on the amino acids. Methods: 25 mmol/L hydroxyproline and 25 mmol/L histidine were incubated with 50 μL Fe3+ and Cu2+ ions at pH 7.4 and 37 °C for 30 mins in separate test tubes. Then 500 μL of 1% thiobarbituricacid (TBA) was added to the incubated amino acids followed by addition of 500 μL of glacial acetic acid. The resultant mixture was vortexed and heated at 100 °C for 30 min. Absorbance readings were noted after cooling to room temperature. The experiment was repeated in the presence of various reagents, like hydroxyl radical scavengers, antioxidant enzymes, and reducing agents and metal ion chelators. Results: The pink chromogen formed with the absorbance maxima at 524 nm, AND shifted to 560 nm in alkaline pH. The absorbance was expressed as TBA-adduct in MDA units. The TBA-adduct decreased in the presence of reducing agents and metal ion chelators. Antioxidant enzymes and hydroxyl radical scavengers did not show any effect. Conclusion: Transitional metal ions in their oxidized state showed significant damage to amino acids, hydroxyproline and histidine. The results indicate the possible role played by high-valent oxo-iron species, ferryl and perferry radicals in damaging biomolecules.

UR - http://www.scopus.com/inward/record.url?scp=77950676973&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950676973&partnerID=8YFLogxK

U2 - 10.1016/S1995-7645(10)60027-2

DO - 10.1016/S1995-7645(10)60027-2

M3 - Article

VL - 3

SP - 32

EP - 35

JO - Asian Pacific Journal of Tropical Medicine

JF - Asian Pacific Journal of Tropical Medicine

SN - 1995-7645

IS - 1

ER -