Transplantation of Hippocampal Cell Lines Restore Spatial Learning in Rats With Ventral Subicular Lesions

J. Rekha, Sridhara Chakravarthy, L. R. Veena, Vani P. Kalai, Rupam Choudhury, Harsha N. Halahalli, Phalguni Anand Alladi, Anandh Dhanushkodi, M. Nirmala, Geetha M. Swamilingiah, Maulishree Agrahari, T. R. Raju, M. M. Panicker, Bindu M. Kutty

Research output: Contribution to journalArticle

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Abstract

We have demonstrated in our previous studies that ventral subicular lesion induces neurodegeneration of the hippocampus and produces cognitive impairment in rats. In the present study, the efficacy of transplanted green fluorescent protein (GFP)-labeled hippocampal cell line (H3-GFP) cells in establishing functional recovery in ventral subicular lesioned rats has been evaluated. The survival of H3-GFP transplants and their ability to express trophic factors in vivo were also investigated. Adult male Wistar rats were subjected to selective lesioning of ventral subiculum and were transplanted with H3-GFP cells into the cornu ammonis 1 (CA1) hippocampus. The transplants settled mainly in the dentate gyrus and expressed neurotrophic factors, brain-derived neurotrophic factor (BDNF), and basic fibroblast growth factor (bFGF). The ventral subicular lesioned (VSL) rats with H3-GFP transplants showed enhanced expression of BDNF in the hippocampus and performed well in eight-arm radial maze and Morris water maze tasks. The VSL rats without hippocampal transplants continued to show cognitive impairment in task learning. The present study demonstrated the H3-GFP transplants mediated recovery of cognitive functions in VSL rats. Our study supports the notion of graft meditated host regeneration and functional recovery through trophic support, although these mechanisms require further investigation.

Original languageEnglish
Pages (from-to)1197-1217
Number of pages21
JournalBehavioral Neuroscience
Volume123
Issue number6
DOIs
Publication statusPublished - 12-2009

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Green Fluorescent Proteins
Transplantation
Hippocampus
Transplants
Cell Line
Brain-Derived Neurotrophic Factor
Aptitude
Recovery of Function
Dentate Gyrus
Nerve Growth Factors
Fibroblast Growth Factor 2
Cognition
Spatial Learning
Wistar Rats
Regeneration
Arm
Learning
Water

All Science Journal Classification (ASJC) codes

  • Behavioral Neuroscience

Cite this

Rekha, J., Chakravarthy, S., Veena, L. R., Kalai, V. P., Choudhury, R., Halahalli, H. N., ... Kutty, B. M. (2009). Transplantation of Hippocampal Cell Lines Restore Spatial Learning in Rats With Ventral Subicular Lesions. Behavioral Neuroscience, 123(6), 1197-1217. https://doi.org/10.1037/a0017655
Rekha, J. ; Chakravarthy, Sridhara ; Veena, L. R. ; Kalai, Vani P. ; Choudhury, Rupam ; Halahalli, Harsha N. ; Alladi, Phalguni Anand ; Dhanushkodi, Anandh ; Nirmala, M. ; Swamilingiah, Geetha M. ; Agrahari, Maulishree ; Raju, T. R. ; Panicker, M. M. ; Kutty, Bindu M. / Transplantation of Hippocampal Cell Lines Restore Spatial Learning in Rats With Ventral Subicular Lesions. In: Behavioral Neuroscience. 2009 ; Vol. 123, No. 6. pp. 1197-1217.
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Rekha, J, Chakravarthy, S, Veena, LR, Kalai, VP, Choudhury, R, Halahalli, HN, Alladi, PA, Dhanushkodi, A, Nirmala, M, Swamilingiah, GM, Agrahari, M, Raju, TR, Panicker, MM & Kutty, BM 2009, 'Transplantation of Hippocampal Cell Lines Restore Spatial Learning in Rats With Ventral Subicular Lesions', Behavioral Neuroscience, vol. 123, no. 6, pp. 1197-1217. https://doi.org/10.1037/a0017655

Transplantation of Hippocampal Cell Lines Restore Spatial Learning in Rats With Ventral Subicular Lesions. / Rekha, J.; Chakravarthy, Sridhara; Veena, L. R.; Kalai, Vani P.; Choudhury, Rupam; Halahalli, Harsha N.; Alladi, Phalguni Anand; Dhanushkodi, Anandh; Nirmala, M.; Swamilingiah, Geetha M.; Agrahari, Maulishree; Raju, T. R.; Panicker, M. M.; Kutty, Bindu M.

In: Behavioral Neuroscience, Vol. 123, No. 6, 12.2009, p. 1197-1217.

Research output: Contribution to journalArticle

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AU - Rekha, J.

AU - Chakravarthy, Sridhara

AU - Veena, L. R.

AU - Kalai, Vani P.

AU - Choudhury, Rupam

AU - Halahalli, Harsha N.

AU - Alladi, Phalguni Anand

AU - Dhanushkodi, Anandh

AU - Nirmala, M.

AU - Swamilingiah, Geetha M.

AU - Agrahari, Maulishree

AU - Raju, T. R.

AU - Panicker, M. M.

AU - Kutty, Bindu M.

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Rekha J, Chakravarthy S, Veena LR, Kalai VP, Choudhury R, Halahalli HN et al. Transplantation of Hippocampal Cell Lines Restore Spatial Learning in Rats With Ventral Subicular Lesions. Behavioral Neuroscience. 2009 Dec;123(6):1197-1217. https://doi.org/10.1037/a0017655