Treatment with Alstonia scholaris Enhances Radiosensitivity In vitro and In vivo

Ganesh Chandra Jagetia, Manjeshwar Shrinath Baliga

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The radiosensitizing effect of 5 μg/mL of alkaloid fraction of Alstonia scholaris (ASERS) was evaluated in various neoplastic cell lines, namely: HeLa, HePG2, HL60, MCF-7, and KB exposed to 0, 0.5, 1, 2, 3, and 4 Gy of γ-radiation. The irradiation of various cells caused a dose-dependent elevation in the cytotoxicity, and a maximum cytotoxic effect was observed at 4 Gy (the highest dose) in all the cell lines studied. The ASERS pretreatment increased the effect of radiation as evidenced by enhanced cell killing when compared with the concurrent phosphate-buffered saline (PBS) treated irradiation group. The greatest elevation in cell killing was observed for HeLa and KB cells, followed by HL60, MCF7, and HePG2 cells. The in vitro observations were confirmed by in vivo studies, where the Ehrlich ascites carcinoma (EAC) bearing mice were treated with 120 mg/kg body weight of ASERS before exposure to 0, 1, 2, 4, 6, and 8 Gy of hemibody (below the rib cage) γ-radiation. Irradiation of EAC mice caused a dose-dependent tumor regression, as evidenced by increased life span of the animals. The pretreatment of tumor-bearing animals with 120 mg/kg ASERS resulted in a further remission in the tumor when compared with the concurrent nondrug-treated irradiated controls; as a result there was a radiation dose-dependent increase in the life span of tumor-bearing animals receiving 120 mg/kg ASERS, except for 8 Gy, where it was less than the concurrent control. The above findings corroborate with a time-dependent decrease in the glutathione (GSH) contents, accompanied by an increase in lipid peroxidation. Our study demonstrates that ASERS treatment enhances the effect of radiation and results in disease-free survival of the mice.

Original languageEnglish
Pages (from-to)917-929
Number of pages13
JournalCancer Biotherapy and Radiopharmaceuticals
Volume18
Issue number6
Publication statusPublished - 01-12-2003
Externally publishedYes

Fingerprint

Alstonia
Radiation Tolerance
Radiation Effects
Ascites
Neoplasms
Radiation
Radiation-Sensitizing Agents
Carcinoma
KB Cells
Radiation Dosage
Cell Line
MCF-7 Cells
Alkaloids
Lipid Peroxidation
Disease-Free Survival
Glutathione
Phosphates
Body Weight
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Pharmacology
  • Oncology

Cite this

Jagetia, Ganesh Chandra ; Baliga, Manjeshwar Shrinath. / Treatment with Alstonia scholaris Enhances Radiosensitivity In vitro and In vivo. In: Cancer Biotherapy and Radiopharmaceuticals. 2003 ; Vol. 18, No. 6. pp. 917-929.
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Treatment with Alstonia scholaris Enhances Radiosensitivity In vitro and In vivo. / Jagetia, Ganesh Chandra; Baliga, Manjeshwar Shrinath.

In: Cancer Biotherapy and Radiopharmaceuticals, Vol. 18, No. 6, 01.12.2003, p. 917-929.

Research output: Contribution to journalArticle

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