Tumour-associated tissue eosinophilia in oral squamous cell carcinoma- A boon or a bane?

Shweta Yellapurkar, Srikant Natarajan, Karen Boaz, Mohan Baliga, Premalatha Shetty, Nidhi Manaktala, Mukul Prasad, Mahalakshmi Ravi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Introduction: The infiltration of tumour stroma by eosinophils, Tumour-Associated Tissue Eosinophilia (TATE) is known to modulate the evolution of Oral Squamous Cell Carcinoma (OSCC). Identification of eosinophils in the inflammatory stroma has been proven to be an important factor in prognostication of malignant tumours including cancers of mouth, oesophagus, larynx, pharynx, breast, lung, intestine and genitourinary tract. Aim: Our study aimed to assess the role of TATE as a prognosticator in OSCC as visualized by Haematoxylin and Eosin (H&E) and congo red staining. Materials and Methods: Thirty histologically-proven cases of OSCC were retrieved from the archives of Department of Oral Pathology, Manipal College of Dental Sciences, Mangalore, Manipal University, Karnataka, India. Two serial sections of 4μm thickness were made and subjected to routine staining with H&E and modified congo red staining, where eosinophil granules stained red and nuclei stained blue. In 40× magnification, 10 HPF at invasive tumour front were assessed for counting eosinophils by placing a 49 square grid (measuring 0.0289 sq mm). Statistical Analysis: The TATE was compared with the prognosticators using Mann-Whitney U-test. The grades of carcinoma were correlated with TATE using Kruskal-Wallis test followed by Post-hoc Bonferronis correction. Agreement of the number of eosinophils counted in the two staining techniques (H&E and Congo red) in OSCC was achieved using interclass correlation coefficient, and Friedman’s test. A value of p< 0.05 was considered statistically significant. Results: Our results showed that tissue eosinophil counts were higher in well-differentiated cases of OSCC, cases with lymph node involvement, decreased survival, without margin involvement and in cases that did not recur. H&E stain showed significantly better visualization of eosinophils resulting in higher eosinophil counts than when seen with Congo red (p=0.008). Conclusion: Thus, TATE can be used as a surrogate marker in prediction of survival and recurrence in OSCC. H&E proved to be a better stain for evaluation of eosinophils.

Original languageEnglish
Pages (from-to)ZC65-ZC68
JournalJournal of Clinical and Diagnostic Research
Volume10
Issue number4
DOIs
Publication statusPublished - 01-04-2016

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Eosinophilia
Eosinophils
Tumors
Squamous Cell Carcinoma
Tissue
Congo Red
Neoplasms
Staining and Labeling
Coloring Agents
Oral Pathology
Red Nucleus
Epithelial Cells
Pathology
Hematoxylin
Eosine Yellowish-(YS)
Mouth Neoplasms
Infiltration
Esophageal Neoplasms
Larynx
Nonparametric Statistics

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Yellapurkar, Shweta ; Natarajan, Srikant ; Boaz, Karen ; Baliga, Mohan ; Shetty, Premalatha ; Manaktala, Nidhi ; Prasad, Mukul ; Ravi, Mahalakshmi. / Tumour-associated tissue eosinophilia in oral squamous cell carcinoma- A boon or a bane?. In: Journal of Clinical and Diagnostic Research. 2016 ; Vol. 10, No. 4. pp. ZC65-ZC68.
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abstract = "Introduction: The infiltration of tumour stroma by eosinophils, Tumour-Associated Tissue Eosinophilia (TATE) is known to modulate the evolution of Oral Squamous Cell Carcinoma (OSCC). Identification of eosinophils in the inflammatory stroma has been proven to be an important factor in prognostication of malignant tumours including cancers of mouth, oesophagus, larynx, pharynx, breast, lung, intestine and genitourinary tract. Aim: Our study aimed to assess the role of TATE as a prognosticator in OSCC as visualized by Haematoxylin and Eosin (H&E) and congo red staining. Materials and Methods: Thirty histologically-proven cases of OSCC were retrieved from the archives of Department of Oral Pathology, Manipal College of Dental Sciences, Mangalore, Manipal University, Karnataka, India. Two serial sections of 4μm thickness were made and subjected to routine staining with H&E and modified congo red staining, where eosinophil granules stained red and nuclei stained blue. In 40× magnification, 10 HPF at invasive tumour front were assessed for counting eosinophils by placing a 49 square grid (measuring 0.0289 sq mm). Statistical Analysis: The TATE was compared with the prognosticators using Mann-Whitney U-test. The grades of carcinoma were correlated with TATE using Kruskal-Wallis test followed by Post-hoc Bonferronis correction. Agreement of the number of eosinophils counted in the two staining techniques (H&E and Congo red) in OSCC was achieved using interclass correlation coefficient, and Friedman’s test. A value of p< 0.05 was considered statistically significant. Results: Our results showed that tissue eosinophil counts were higher in well-differentiated cases of OSCC, cases with lymph node involvement, decreased survival, without margin involvement and in cases that did not recur. H&E stain showed significantly better visualization of eosinophils resulting in higher eosinophil counts than when seen with Congo red (p=0.008). Conclusion: Thus, TATE can be used as a surrogate marker in prediction of survival and recurrence in OSCC. H&E proved to be a better stain for evaluation of eosinophils.",
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Tumour-associated tissue eosinophilia in oral squamous cell carcinoma- A boon or a bane? / Yellapurkar, Shweta; Natarajan, Srikant; Boaz, Karen; Baliga, Mohan; Shetty, Premalatha; Manaktala, Nidhi; Prasad, Mukul; Ravi, Mahalakshmi.

In: Journal of Clinical and Diagnostic Research, Vol. 10, No. 4, 01.04.2016, p. ZC65-ZC68.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Tumour-associated tissue eosinophilia in oral squamous cell carcinoma- A boon or a bane?

AU - Yellapurkar, Shweta

AU - Natarajan, Srikant

AU - Boaz, Karen

AU - Baliga, Mohan

AU - Shetty, Premalatha

AU - Manaktala, Nidhi

AU - Prasad, Mukul

AU - Ravi, Mahalakshmi

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Introduction: The infiltration of tumour stroma by eosinophils, Tumour-Associated Tissue Eosinophilia (TATE) is known to modulate the evolution of Oral Squamous Cell Carcinoma (OSCC). Identification of eosinophils in the inflammatory stroma has been proven to be an important factor in prognostication of malignant tumours including cancers of mouth, oesophagus, larynx, pharynx, breast, lung, intestine and genitourinary tract. Aim: Our study aimed to assess the role of TATE as a prognosticator in OSCC as visualized by Haematoxylin and Eosin (H&E) and congo red staining. Materials and Methods: Thirty histologically-proven cases of OSCC were retrieved from the archives of Department of Oral Pathology, Manipal College of Dental Sciences, Mangalore, Manipal University, Karnataka, India. Two serial sections of 4μm thickness were made and subjected to routine staining with H&E and modified congo red staining, where eosinophil granules stained red and nuclei stained blue. In 40× magnification, 10 HPF at invasive tumour front were assessed for counting eosinophils by placing a 49 square grid (measuring 0.0289 sq mm). Statistical Analysis: The TATE was compared with the prognosticators using Mann-Whitney U-test. The grades of carcinoma were correlated with TATE using Kruskal-Wallis test followed by Post-hoc Bonferronis correction. Agreement of the number of eosinophils counted in the two staining techniques (H&E and Congo red) in OSCC was achieved using interclass correlation coefficient, and Friedman’s test. A value of p< 0.05 was considered statistically significant. Results: Our results showed that tissue eosinophil counts were higher in well-differentiated cases of OSCC, cases with lymph node involvement, decreased survival, without margin involvement and in cases that did not recur. H&E stain showed significantly better visualization of eosinophils resulting in higher eosinophil counts than when seen with Congo red (p=0.008). Conclusion: Thus, TATE can be used as a surrogate marker in prediction of survival and recurrence in OSCC. H&E proved to be a better stain for evaluation of eosinophils.

AB - Introduction: The infiltration of tumour stroma by eosinophils, Tumour-Associated Tissue Eosinophilia (TATE) is known to modulate the evolution of Oral Squamous Cell Carcinoma (OSCC). Identification of eosinophils in the inflammatory stroma has been proven to be an important factor in prognostication of malignant tumours including cancers of mouth, oesophagus, larynx, pharynx, breast, lung, intestine and genitourinary tract. Aim: Our study aimed to assess the role of TATE as a prognosticator in OSCC as visualized by Haematoxylin and Eosin (H&E) and congo red staining. Materials and Methods: Thirty histologically-proven cases of OSCC were retrieved from the archives of Department of Oral Pathology, Manipal College of Dental Sciences, Mangalore, Manipal University, Karnataka, India. Two serial sections of 4μm thickness were made and subjected to routine staining with H&E and modified congo red staining, where eosinophil granules stained red and nuclei stained blue. In 40× magnification, 10 HPF at invasive tumour front were assessed for counting eosinophils by placing a 49 square grid (measuring 0.0289 sq mm). Statistical Analysis: The TATE was compared with the prognosticators using Mann-Whitney U-test. The grades of carcinoma were correlated with TATE using Kruskal-Wallis test followed by Post-hoc Bonferronis correction. Agreement of the number of eosinophils counted in the two staining techniques (H&E and Congo red) in OSCC was achieved using interclass correlation coefficient, and Friedman’s test. A value of p< 0.05 was considered statistically significant. Results: Our results showed that tissue eosinophil counts were higher in well-differentiated cases of OSCC, cases with lymph node involvement, decreased survival, without margin involvement and in cases that did not recur. H&E stain showed significantly better visualization of eosinophils resulting in higher eosinophil counts than when seen with Congo red (p=0.008). Conclusion: Thus, TATE can be used as a surrogate marker in prediction of survival and recurrence in OSCC. H&E proved to be a better stain for evaluation of eosinophils.

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