Types of advanced optical microscopy techniques for breast cancer research: a review

Aparna Dravid U, Nirmal Mazumder

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A cancerous cell is characterized by morphological and metabolic changes which are the key features of carcinogenesis. Adenosine triphosphate (ATP) in cancer cells is primarily produced by aerobic glycolysis rather than oxidative phosphorylation. In normal cellular metabolism, nicotinamide adenine dinucleotide (NADH) is considered as a principle electron donor and flavin adenine dinucleotide (FAD) as an electron acceptor. During metabolism in a cancerous cell, a net increase in NADH is found as the pathway switched from oxidative phosphorylation to aerobic glycolysis. Often during initiation and progression of cancer, the developmental regulation of extracellular matrix (ECM) is restricted and becomes disorganized. Tumor cell behavior is regulated by the ECM in the tumor micro environment. Collagen, which forms the scaffold of tumor micro-environment also influences its behavior. Advanced optical microscopy techniques are useful for determining the metabolic characteristics of cancerous, normal cells and tissues. They can be used to identify the collagen microstructure and the function of NADH, FAD, and lipids in living system. In this review article, various optical microscopy techniques applied for breast cancer research are discussed including fluorescence, confocal, second harmonic generation (SHG), coherent anti-Stokes Raman scattering (CARS), and fluorescence lifetime imaging (FLIM).

Original languageEnglish
JournalLasers in Medical Science
DOIs
Publication statusPublished - 01-01-2018

Fingerprint

Microscopy
NAD
Breast Neoplasms
Research
Flavin-Adenine Dinucleotide
Oxidative Phosphorylation
Glycolysis
Neoplasms
Extracellular Matrix
Collagen
Electrons
Raman Spectrum Analysis
Optical Imaging
Carcinogenesis
Adenosine Triphosphate
Fluorescence
Lipids

All Science Journal Classification (ASJC) codes

  • Surgery
  • Dermatology

Cite this

@article{bc9c3208e6af461b84f54bb6ebd27f3c,
title = "Types of advanced optical microscopy techniques for breast cancer research: a review",
abstract = "A cancerous cell is characterized by morphological and metabolic changes which are the key features of carcinogenesis. Adenosine triphosphate (ATP) in cancer cells is primarily produced by aerobic glycolysis rather than oxidative phosphorylation. In normal cellular metabolism, nicotinamide adenine dinucleotide (NADH) is considered as a principle electron donor and flavin adenine dinucleotide (FAD) as an electron acceptor. During metabolism in a cancerous cell, a net increase in NADH is found as the pathway switched from oxidative phosphorylation to aerobic glycolysis. Often during initiation and progression of cancer, the developmental regulation of extracellular matrix (ECM) is restricted and becomes disorganized. Tumor cell behavior is regulated by the ECM in the tumor micro environment. Collagen, which forms the scaffold of tumor micro-environment also influences its behavior. Advanced optical microscopy techniques are useful for determining the metabolic characteristics of cancerous, normal cells and tissues. They can be used to identify the collagen microstructure and the function of NADH, FAD, and lipids in living system. In this review article, various optical microscopy techniques applied for breast cancer research are discussed including fluorescence, confocal, second harmonic generation (SHG), coherent anti-Stokes Raman scattering (CARS), and fluorescence lifetime imaging (FLIM).",
author = "{Dravid U}, Aparna and Nirmal Mazumder",
year = "2018",
month = "1",
day = "1",
doi = "10.1007/s10103-018-2659-6",
language = "English",
journal = "Lasers in Medical Science",
issn = "0268-8921",
publisher = "Springer London",

}

Types of advanced optical microscopy techniques for breast cancer research : a review. / Dravid U, Aparna; Mazumder, Nirmal.

In: Lasers in Medical Science, 01.01.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Types of advanced optical microscopy techniques for breast cancer research

T2 - a review

AU - Dravid U, Aparna

AU - Mazumder, Nirmal

PY - 2018/1/1

Y1 - 2018/1/1

N2 - A cancerous cell is characterized by morphological and metabolic changes which are the key features of carcinogenesis. Adenosine triphosphate (ATP) in cancer cells is primarily produced by aerobic glycolysis rather than oxidative phosphorylation. In normal cellular metabolism, nicotinamide adenine dinucleotide (NADH) is considered as a principle electron donor and flavin adenine dinucleotide (FAD) as an electron acceptor. During metabolism in a cancerous cell, a net increase in NADH is found as the pathway switched from oxidative phosphorylation to aerobic glycolysis. Often during initiation and progression of cancer, the developmental regulation of extracellular matrix (ECM) is restricted and becomes disorganized. Tumor cell behavior is regulated by the ECM in the tumor micro environment. Collagen, which forms the scaffold of tumor micro-environment also influences its behavior. Advanced optical microscopy techniques are useful for determining the metabolic characteristics of cancerous, normal cells and tissues. They can be used to identify the collagen microstructure and the function of NADH, FAD, and lipids in living system. In this review article, various optical microscopy techniques applied for breast cancer research are discussed including fluorescence, confocal, second harmonic generation (SHG), coherent anti-Stokes Raman scattering (CARS), and fluorescence lifetime imaging (FLIM).

AB - A cancerous cell is characterized by morphological and metabolic changes which are the key features of carcinogenesis. Adenosine triphosphate (ATP) in cancer cells is primarily produced by aerobic glycolysis rather than oxidative phosphorylation. In normal cellular metabolism, nicotinamide adenine dinucleotide (NADH) is considered as a principle electron donor and flavin adenine dinucleotide (FAD) as an electron acceptor. During metabolism in a cancerous cell, a net increase in NADH is found as the pathway switched from oxidative phosphorylation to aerobic glycolysis. Often during initiation and progression of cancer, the developmental regulation of extracellular matrix (ECM) is restricted and becomes disorganized. Tumor cell behavior is regulated by the ECM in the tumor micro environment. Collagen, which forms the scaffold of tumor micro-environment also influences its behavior. Advanced optical microscopy techniques are useful for determining the metabolic characteristics of cancerous, normal cells and tissues. They can be used to identify the collagen microstructure and the function of NADH, FAD, and lipids in living system. In this review article, various optical microscopy techniques applied for breast cancer research are discussed including fluorescence, confocal, second harmonic generation (SHG), coherent anti-Stokes Raman scattering (CARS), and fluorescence lifetime imaging (FLIM).

UR - http://www.scopus.com/inward/record.url?scp=85055130632&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055130632&partnerID=8YFLogxK

U2 - 10.1007/s10103-018-2659-6

DO - 10.1007/s10103-018-2659-6

M3 - Article

AN - SCOPUS:85055130632

JO - Lasers in Medical Science

JF - Lasers in Medical Science

SN - 0268-8921

ER -