Upregulation of TFAM and mitochondria copy number in human lymphoblastoid cells

Sanjiban Chakrabarty, Reena Reshma D'Souza, Shama Prasada Kabekkodu, Puthiya M. Gopinath, Rodrigue Rossignol, Kapaettu Satyamoorthy

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Mitochondria are central to several physiological and pathological conditions in humans. In the present study, we performed copy number analysis of nuclear encoded mitochondrial genes, in peripheral blood mononuclear cells (PBMCs) and its representative lymphoblastoid cells (LCLs). We have observed hyper diploid copies of mitochondrial transcription factor A (TFAM) gene in the LCLs along with increased mtDNA copy number, mitochondrial mass, intracellular ROS and mitochondrial membrane potential, suggesting elevated mitochondrial biogenesis in LCLs. Gene expression analysis confirmed TFAM over-expression in LCLs when compared to PBMC. Based on our observation, we suggest that increased copy number of TFAM gene upregulates its expression, increases mtDNA copy numbers and protects it from oxidative stress induced damage in the transformed LCLs.

Original languageEnglish
Pages (from-to)52-58
Number of pages7
JournalMitochondrion
Volume15
Issue number1
DOIs
Publication statusPublished - 2014

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Mitochondria
Up-Regulation
Mitochondrial DNA
Blood Cells
Intracellular Membranes
Mitochondrial Genes
Gene Dosage
Mitochondrial Membrane Potential
Organelle Biogenesis
Diploidy
Oxidative Stress
Observation
Gene Expression
Genes

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Chakrabarty, Sanjiban ; D'Souza, Reena Reshma ; Kabekkodu, Shama Prasada ; Gopinath, Puthiya M. ; Rossignol, Rodrigue ; Satyamoorthy, Kapaettu. / Upregulation of TFAM and mitochondria copy number in human lymphoblastoid cells. In: Mitochondrion. 2014 ; Vol. 15, No. 1. pp. 52-58.
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Upregulation of TFAM and mitochondria copy number in human lymphoblastoid cells. / Chakrabarty, Sanjiban; D'Souza, Reena Reshma; Kabekkodu, Shama Prasada; Gopinath, Puthiya M.; Rossignol, Rodrigue; Satyamoorthy, Kapaettu.

In: Mitochondrion, Vol. 15, No. 1, 2014, p. 52-58.

Research output: Contribution to journalArticle

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AU - Chakrabarty, Sanjiban

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AB - Mitochondria are central to several physiological and pathological conditions in humans. In the present study, we performed copy number analysis of nuclear encoded mitochondrial genes, in peripheral blood mononuclear cells (PBMCs) and its representative lymphoblastoid cells (LCLs). We have observed hyper diploid copies of mitochondrial transcription factor A (TFAM) gene in the LCLs along with increased mtDNA copy number, mitochondrial mass, intracellular ROS and mitochondrial membrane potential, suggesting elevated mitochondrial biogenesis in LCLs. Gene expression analysis confirmed TFAM over-expression in LCLs when compared to PBMC. Based on our observation, we suggest that increased copy number of TFAM gene upregulates its expression, increases mtDNA copy numbers and protects it from oxidative stress induced damage in the transformed LCLs.

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