Upregulation of TFAM and mitochondria copy number in human lymphoblastoid cells

Sanjiban Chakrabarty, Reena Reshma D'Souza, Shama Prasada Kabekkodu, Puthiya M. Gopinath, Rodrigue Rossignol, Kapaettu Satyamoorthy

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14 Citations (Scopus)

Abstract

Mitochondria are central to several physiological and pathological conditions in humans. In the present study, we performed copy number analysis of nuclear encoded mitochondrial genes, in peripheral blood mononuclear cells (PBMCs) and its representative lymphoblastoid cells (LCLs). We have observed hyper diploid copies of mitochondrial transcription factor A (TFAM) gene in the LCLs along with increased mtDNA copy number, mitochondrial mass, intracellular ROS and mitochondrial membrane potential, suggesting elevated mitochondrial biogenesis in LCLs. Gene expression analysis confirmed TFAM over-expression in LCLs when compared to PBMC. Based on our observation, we suggest that increased copy number of TFAM gene upregulates its expression, increases mtDNA copy numbers and protects it from oxidative stress induced damage in the transformed LCLs.

Original languageEnglish
Pages (from-to)52-58
Number of pages7
JournalMitochondrion
Volume15
Issue number1
DOIs
Publication statusPublished - 2014

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All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

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