Use of multicellular tumor spheroids to dissect endothelial cell-tumor cell interactions

A role for T-cadherin in tumor angiogenesis

Sourabh Ghosh, Manjunath B. Joshi, Danila Ivanov, Chantal Feder-Mengus, Giulio C. Spagnoli, Ivan Martin, Paul Erne, Therese J. Resink

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

This study addresses establishment of an "in vitro" melanoma angiogenesis model using multicellular tumor spheroids (MCTS) of differentiated (HBL) or undifferentiated (NA8) melanoma cell lines. DNA microarray assay and qRT-PCR indicated upregulation of pro-angiogenic factors IL-8, VEGF, Ephrin A1 and ANGPTL4 in NA8-MCTSs (vs. monolayers) whereas these were absent in MCTS and monolayer cultures of HBL. Upon co-culture with endothelial cell line HMEC-1 NA8-MCTS attract, whereas HBL-MCTS repulse, HMEC-1. Overexpression of T-cadherin in HMEC-1 leads to their increased invasion and network formation within NA8-MCTS. Given an appropriate angiogenic tumor microenvironment, T-cadherin upregulation on endothelial cells may potentiate intratumoral angiogenesis.

Original languageEnglish
Pages (from-to)4523-4528
Number of pages6
JournalFEBS Letters
Volume581
Issue number23
DOIs
Publication statusPublished - 18-09-2007

Fingerprint

Cellular Spheroids
Endothelial cells
Cell Communication
Tumors
Endothelial Cells
Cells
Neoplasms
Ephrin-A1
Melanoma
Cell culture
Up-Regulation
Monolayers
Cell Line
Tumor Microenvironment
Angiogenesis Inducing Agents
Coculture Techniques
Oligonucleotide Array Sequence Analysis
Interleukin-8
Vascular Endothelial Growth Factor A
Microarrays

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Ghosh, Sourabh ; Joshi, Manjunath B. ; Ivanov, Danila ; Feder-Mengus, Chantal ; Spagnoli, Giulio C. ; Martin, Ivan ; Erne, Paul ; Resink, Therese J. / Use of multicellular tumor spheroids to dissect endothelial cell-tumor cell interactions : A role for T-cadherin in tumor angiogenesis. In: FEBS Letters. 2007 ; Vol. 581, No. 23. pp. 4523-4528.
@article{ad7034f038cb4cf2bd508bf868cc9b03,
title = "Use of multicellular tumor spheroids to dissect endothelial cell-tumor cell interactions: A role for T-cadherin in tumor angiogenesis",
abstract = "This study addresses establishment of an {"}in vitro{"} melanoma angiogenesis model using multicellular tumor spheroids (MCTS) of differentiated (HBL) or undifferentiated (NA8) melanoma cell lines. DNA microarray assay and qRT-PCR indicated upregulation of pro-angiogenic factors IL-8, VEGF, Ephrin A1 and ANGPTL4 in NA8-MCTSs (vs. monolayers) whereas these were absent in MCTS and monolayer cultures of HBL. Upon co-culture with endothelial cell line HMEC-1 NA8-MCTS attract, whereas HBL-MCTS repulse, HMEC-1. Overexpression of T-cadherin in HMEC-1 leads to their increased invasion and network formation within NA8-MCTS. Given an appropriate angiogenic tumor microenvironment, T-cadherin upregulation on endothelial cells may potentiate intratumoral angiogenesis.",
author = "Sourabh Ghosh and Joshi, {Manjunath B.} and Danila Ivanov and Chantal Feder-Mengus and Spagnoli, {Giulio C.} and Ivan Martin and Paul Erne and Resink, {Therese J.}",
year = "2007",
month = "9",
day = "18",
doi = "10.1016/j.febslet.2007.08.038",
language = "English",
volume = "581",
pages = "4523--4528",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "23",

}

Use of multicellular tumor spheroids to dissect endothelial cell-tumor cell interactions : A role for T-cadherin in tumor angiogenesis. / Ghosh, Sourabh; Joshi, Manjunath B.; Ivanov, Danila; Feder-Mengus, Chantal; Spagnoli, Giulio C.; Martin, Ivan; Erne, Paul; Resink, Therese J.

In: FEBS Letters, Vol. 581, No. 23, 18.09.2007, p. 4523-4528.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Use of multicellular tumor spheroids to dissect endothelial cell-tumor cell interactions

T2 - A role for T-cadherin in tumor angiogenesis

AU - Ghosh, Sourabh

AU - Joshi, Manjunath B.

AU - Ivanov, Danila

AU - Feder-Mengus, Chantal

AU - Spagnoli, Giulio C.

AU - Martin, Ivan

AU - Erne, Paul

AU - Resink, Therese J.

PY - 2007/9/18

Y1 - 2007/9/18

N2 - This study addresses establishment of an "in vitro" melanoma angiogenesis model using multicellular tumor spheroids (MCTS) of differentiated (HBL) or undifferentiated (NA8) melanoma cell lines. DNA microarray assay and qRT-PCR indicated upregulation of pro-angiogenic factors IL-8, VEGF, Ephrin A1 and ANGPTL4 in NA8-MCTSs (vs. monolayers) whereas these were absent in MCTS and monolayer cultures of HBL. Upon co-culture with endothelial cell line HMEC-1 NA8-MCTS attract, whereas HBL-MCTS repulse, HMEC-1. Overexpression of T-cadherin in HMEC-1 leads to their increased invasion and network formation within NA8-MCTS. Given an appropriate angiogenic tumor microenvironment, T-cadherin upregulation on endothelial cells may potentiate intratumoral angiogenesis.

AB - This study addresses establishment of an "in vitro" melanoma angiogenesis model using multicellular tumor spheroids (MCTS) of differentiated (HBL) or undifferentiated (NA8) melanoma cell lines. DNA microarray assay and qRT-PCR indicated upregulation of pro-angiogenic factors IL-8, VEGF, Ephrin A1 and ANGPTL4 in NA8-MCTSs (vs. monolayers) whereas these were absent in MCTS and monolayer cultures of HBL. Upon co-culture with endothelial cell line HMEC-1 NA8-MCTS attract, whereas HBL-MCTS repulse, HMEC-1. Overexpression of T-cadherin in HMEC-1 leads to their increased invasion and network formation within NA8-MCTS. Given an appropriate angiogenic tumor microenvironment, T-cadherin upregulation on endothelial cells may potentiate intratumoral angiogenesis.

UR - http://www.scopus.com/inward/record.url?scp=34548410347&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548410347&partnerID=8YFLogxK

U2 - 10.1016/j.febslet.2007.08.038

DO - 10.1016/j.febslet.2007.08.038

M3 - Article

VL - 581

SP - 4523

EP - 4528

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 23

ER -