Validation of a sensitive and robust reversed phase-HPLC method for determination of temozolomide

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Temozolomide is a physiologically pH labile, alkylating agent used in glioblastoma multiforme. An attempt has been made to develop and validate a simple, sensitive, accurate, precise and robust HPLC method for quantification of temozolomide. Ammonium formate and acetonitrile combination used as mobile phase using BDS C18 column resulted in elution of temozolomide with retention time of 5.866 min. The various parameters, precision, accuracy and robustness of the method were evaluated. The % RSD of the parameters were acceptable (< 2 or/and 1%). The LOD and LOQ were found to be 13.13 and 39.81 ng/mL, respectively. The recovery of the drug was observed to be 99.97 to 100.93%.

Original languageEnglish
Pages (from-to)967-971
Number of pages5
JournalLatin American Journal of Pharmacy
Issue number5
Publication statusPublished - 2016


All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Drug Discovery

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