Vincristine increases the genomic instability in irradiated cultured human peripheral blood lymphocytes

Ganesh Chandra Jagetia, Manjeshwar Shrinath Baliga

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Increase in the genomic instability by 10 ng of vincristine (VCR) pretreatment was studied in cultured human peripheral blood lymphocytes exposed to 0, 0.5, 1, 2, and 3 Gy gamma radiation by the micronucleus assay. The frequency of micronucleated binucleate lymphocytes (MNBNC) increased in a dose dependent manner after exposure to different doses of gamma radiation and the dose response was linear. The pre-treatment of lymphocytes with 10 ng/ml VCR caused a further elevation in the frequency of MNBNC and this was significantly greater than that of the concurrent PBS pre-treated irradiated lymphocytes. The dose response for VCR treated group was linear up to 1 Gy irradiation. A further increase in the radiation dose resulted in a decline in the induction of MNBNC in VCR pretreated irradiated group, although it was significantly higher than the PBS treated irradiated group. VCR pretreatment not only increased the frequency of MNBNC with one micronuclei but also the frequency of MNBNC with 2, 3 and 4 MN significantly, indicating the increase in the multiple sites of damage to DNA by VCR pre-treatment. This increase in multiple MNBNC was also dose dependent, however, the dose response was linear quadratic.

Original languageEnglish
Pages (from-to)179-186
Number of pages8
JournalToxicology Letters
Volume126
Issue number3
DOIs
Publication statusPublished - 07-02-2002
Externally publishedYes

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Lymphocytes
Genomic Instability
Vincristine
Blood
Dosimetry
Gamma Rays
Gamma rays
Radiation Dosage
Micronucleus Tests
DNA Damage
Assays
Irradiation
Radiation
DNA

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Jagetia, Ganesh Chandra ; Shrinath Baliga, Manjeshwar. / Vincristine increases the genomic instability in irradiated cultured human peripheral blood lymphocytes. In: Toxicology Letters. 2002 ; Vol. 126, No. 3. pp. 179-186.
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Vincristine increases the genomic instability in irradiated cultured human peripheral blood lymphocytes. / Jagetia, Ganesh Chandra; Shrinath Baliga, Manjeshwar.

In: Toxicology Letters, Vol. 126, No. 3, 07.02.2002, p. 179-186.

Research output: Contribution to journalArticle

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