Withaferin A

A new radiosensitizer from the Indian medicinal plant withania somnifera

P. Uma Devi, K. Akagi, V. Ostapenko, Y. Tanaka, T. Sugahara

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Withaferin A, a steroidal lactone isolated from the roots of the Indian medicinal plant Withania somnifera, reduced survival of V79 cells in a dose-dependent manner. LD50 for survival was 16 μM. One-hour treatment with a nontoxic dose of 2.1 μM before irradiation significantly enhanced cell killing, giving a sensitizer enhancement ratio (SER) of 1.5 for 31% survival and 1.4 for 10% survival. SER increased with drug dose, but at higher doses the increased lethality appears to be due to two effects-drug toxicity and radiosensitization. The drug induced a G2-M block, with a maximum accumulation of cells in G2-M phase at 4h after treatment with 10.5 μM withaferin A for 1 h. The applicability of this drug as a radiosensitizer in cancer therapy needs to be explored.

Original languageEnglish
Pages (from-to)193-197
Number of pages5
JournalInternational Journal of Radiation Biology
Volume69
Issue number2
DOIs
Publication statusPublished - 01-05-1996
Externally publishedYes

Fingerprint

Withania
Medicinal Plants
Pharmaceutical Preparations
G2 Phase
Lethal Dose 50
Lactones
Drug-Related Side Effects and Adverse Reactions
Cell Division
Cell Survival
withaferin A
Neoplasms
Therapeutics

All Science Journal Classification (ASJC) codes

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

Cite this

Uma Devi, P. ; Akagi, K. ; Ostapenko, V. ; Tanaka, Y. ; Sugahara, T. / Withaferin A : A new radiosensitizer from the Indian medicinal plant withania somnifera. In: International Journal of Radiation Biology. 1996 ; Vol. 69, No. 2. pp. 193-197.
@article{5ffeea1623f845be90b4b56201c7d348,
title = "Withaferin A: A new radiosensitizer from the Indian medicinal plant withania somnifera",
abstract = "Withaferin A, a steroidal lactone isolated from the roots of the Indian medicinal plant Withania somnifera, reduced survival of V79 cells in a dose-dependent manner. LD50 for survival was 16 μM. One-hour treatment with a nontoxic dose of 2.1 μM before irradiation significantly enhanced cell killing, giving a sensitizer enhancement ratio (SER) of 1.5 for 31{\%} survival and 1.4 for 10{\%} survival. SER increased with drug dose, but at higher doses the increased lethality appears to be due to two effects-drug toxicity and radiosensitization. The drug induced a G2-M block, with a maximum accumulation of cells in G2-M phase at 4h after treatment with 10.5 μM withaferin A for 1 h. The applicability of this drug as a radiosensitizer in cancer therapy needs to be explored.",
author = "{Uma Devi}, P. and K. Akagi and V. Ostapenko and Y. Tanaka and T. Sugahara",
year = "1996",
month = "5",
day = "1",
doi = "10.1080/095530096146020",
language = "English",
volume = "69",
pages = "193--197",
journal = "International Journal of Radiation Biology",
issn = "0955-3002",
publisher = "Informa Healthcare",
number = "2",

}

Withaferin A : A new radiosensitizer from the Indian medicinal plant withania somnifera. / Uma Devi, P.; Akagi, K.; Ostapenko, V.; Tanaka, Y.; Sugahara, T.

In: International Journal of Radiation Biology, Vol. 69, No. 2, 01.05.1996, p. 193-197.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Withaferin A

T2 - A new radiosensitizer from the Indian medicinal plant withania somnifera

AU - Uma Devi, P.

AU - Akagi, K.

AU - Ostapenko, V.

AU - Tanaka, Y.

AU - Sugahara, T.

PY - 1996/5/1

Y1 - 1996/5/1

N2 - Withaferin A, a steroidal lactone isolated from the roots of the Indian medicinal plant Withania somnifera, reduced survival of V79 cells in a dose-dependent manner. LD50 for survival was 16 μM. One-hour treatment with a nontoxic dose of 2.1 μM before irradiation significantly enhanced cell killing, giving a sensitizer enhancement ratio (SER) of 1.5 for 31% survival and 1.4 for 10% survival. SER increased with drug dose, but at higher doses the increased lethality appears to be due to two effects-drug toxicity and radiosensitization. The drug induced a G2-M block, with a maximum accumulation of cells in G2-M phase at 4h after treatment with 10.5 μM withaferin A for 1 h. The applicability of this drug as a radiosensitizer in cancer therapy needs to be explored.

AB - Withaferin A, a steroidal lactone isolated from the roots of the Indian medicinal plant Withania somnifera, reduced survival of V79 cells in a dose-dependent manner. LD50 for survival was 16 μM. One-hour treatment with a nontoxic dose of 2.1 μM before irradiation significantly enhanced cell killing, giving a sensitizer enhancement ratio (SER) of 1.5 for 31% survival and 1.4 for 10% survival. SER increased with drug dose, but at higher doses the increased lethality appears to be due to two effects-drug toxicity and radiosensitization. The drug induced a G2-M block, with a maximum accumulation of cells in G2-M phase at 4h after treatment with 10.5 μM withaferin A for 1 h. The applicability of this drug as a radiosensitizer in cancer therapy needs to be explored.

UR - http://www.scopus.com/inward/record.url?scp=0029933319&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029933319&partnerID=8YFLogxK

U2 - 10.1080/095530096146020

DO - 10.1080/095530096146020

M3 - Article

VL - 69

SP - 193

EP - 197

JO - International Journal of Radiation Biology

JF - International Journal of Radiation Biology

SN - 0955-3002

IS - 2

ER -