Wound healing activity of NOE-aspirin: A pre-clinical study

Mandeep Kaushal, N. Gopalan Kutty, C. Mallikarjuna Rao

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Aspirin, one of the oldest non-steroidal anti-inflammatory drugs, impedes tissue repair by virtue of retarding inflammation. The present study was undertaken to find out if linking of nitrooxyethyl ester to aspirin reverses its healing-depressant propensity. Nitrooxyethyl ester of aspirin (NOE-Asp) was synthesized in our laboratory through well-established synthetic pathway, starting from aspirin through esterification with ethylene glycol and nitration with a mixture of nitric and sulfuric acids at 0 °C. The effect of NOE-Asp on phases of healing such as collagenation, wound contraction and epithelialization and on scar size of healed wound was evaluated in three wound models-incision, dead space, and excision wounds. To assess its influence on the oxidative stress, the levels of glutathione (GSH) and thiobarbiturate reactive species (TBARS) were also determined in 10-day-old granulation tissue. NOE-Asp was further screened for its anti-inflammatory activity in rat paw edema model. NOE-Asp promoted collagenation (increase in breaking strength, granulation weight, and collagen content), wound contraction, and epithelialization phases of healing. NOE-Asp also showed a significant antioxidant effect in 10-day-old granulation tissue as compared to aspirin. The results vindicate our assumption that the esterification of aspirin with nirooxyethyl group reverses the healing-suppressant effect of aspirin. The compound also showed equipotent anti-inflammatory activity as aspirin.

Original languageEnglish
Pages (from-to)150-156
Number of pages7
JournalNitric Oxide - Biology and Chemistry
Volume16
Issue number1
DOIs
Publication statusPublished - 02-2007

All Science Journal Classification (ASJC) codes

  • Physiology
  • Biochemistry
  • Clinical Biochemistry
  • Cancer Research

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