Wound healing activity of Ocimum sanctum linn with supportive role of antioxidant enzymes

Somashekar Shetty, Saraswati Udupa, Laxminarayana Udupa, Nagabhushana Somayaji

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The present study was performed to evaluate the wound healing and antioxidant effect of aqueous extract of Ocimum sanctum Linn. (O.sanctum) in rats. Albino rats of either sex were divided into 2 groups. Group I: Wounded control rats; Group II: Wounded rats administered O.sanctum aqueous extract. Wound breaking strength in incision wound model, epithelization period and percent wound contraction in excision wound model were studied. Using dead space wound model, granulation tissue breaking strength, granulation tissue dry weight, hydoxyproline level in dry granulation tissue, superoxide dismutase (SOD) and catalase levels in wet granulation tissue were estimated in both the groups. Increased wound breaking strength, decreased epithelization period, increased percent wound contraction, increased granulation tissue weight and hydroxyproline concentrations were observed. The increased activity of antioxidant enzymes such as SOD, catalase level in extract treated group compared to controls. Granulation tissue was subjected to histopathological examination to determine the pattern of lay-down for collagen using Haematoxylin and Eosin stains which confirm the results. Owing to wound healing and antioxidant activities, O.sanctum may be useful in the management of abnormal healing such as keloids and hypertrophic scars.

Original languageEnglish
Pages (from-to)163-168
Number of pages6
JournalIndian Journal of Physiology and Pharmacology
Volume50
Issue number2
Publication statusPublished - 01-04-2006

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Granulation Tissue
Wound Healing
Antioxidants
Wounds and Injuries
Enzymes
Catalase
Superoxide Dismutase
Space Simulation
Hypertrophic Cicatrix
Keloid
Weights and Measures
Hydroxyproline
Hematoxylin
Eosine Yellowish-(YS)
Ocimum sanctum
Coloring Agents
Collagen

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pharmacology
  • Physiology (medical)

Cite this

Shetty, Somashekar ; Udupa, Saraswati ; Udupa, Laxminarayana ; Somayaji, Nagabhushana. / Wound healing activity of Ocimum sanctum linn with supportive role of antioxidant enzymes. In: Indian Journal of Physiology and Pharmacology. 2006 ; Vol. 50, No. 2. pp. 163-168.
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Wound healing activity of Ocimum sanctum linn with supportive role of antioxidant enzymes. / Shetty, Somashekar; Udupa, Saraswati; Udupa, Laxminarayana; Somayaji, Nagabhushana.

In: Indian Journal of Physiology and Pharmacology, Vol. 50, No. 2, 01.04.2006, p. 163-168.

Research output: Contribution to journalArticle

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